Not known Factual Statements About hplc column size
Not known Factual Statements About hplc column size
Blog Article
This website page won't exist in your picked language. Your preference was saved and you may be notified at the time a webpage may be viewed with your language.
Liquid chromatography is one of the three principal branches of chromatography. It entails a little quantity of liquid sample placement right into a tube packed with porous particles.
In certain conditions, coping with challenging molecular approaches is actually Section of the job description. The scientific…
Immediate screening of chromatographic problems is necessary to determine the most beneficial purification conditions. Scouting of conditions is often reached on lab scale chromatography methods or automatic robotic systems.
As an example, a protein that's only somewhat lesser than the usual pore may well enter the pore but will not conveniently go away the moment within.
Environmental Evaluation: Chiral HPLC is used in environmental monitoring to separate and evaluate chiral pollutants, pesticides, together with other compounds that exist as enantiomers.
Increased stress above several injections: This happens when sample components slowly accumulate with the frit or beginning of your column. Retain a logbook of pressures right before and following runs to assist determine these difficulties.
The following video clip here focusing only regarding how to clean your HPLC column by way of reverse flushing. It'd be time to flush your HPLC column any time you’re observing superior back again tension, low efficiency, break up peaks, or the most typical situation, particulate Make-up at The top on the column.
And When you have anymore issues about how to very best take care of your column, operate an analysis, or anything else pertaining to work, Phenomenex provides a free specialized support through Live Chat, which lets you talk with Specialized Authorities 24/7 worldwide. They can assist with Nearly any issue immediately.
The material of our Site is always readily available in English and partly in other languages. Pick your most well-liked language and We'll tell you about the material in that language, if accessible.
Connected or semi-related downstream processing presents sizeable enhancements to productiveness, Value, timelines, and facility footprint. Our flexible portfolio allows you decide on an intensification tactic that aligns with your distinctive process plans.
Offered the infinite range of compounds and structural variety of prospective analytes, HPLC is rarely a a person-size-matches-all approach. From nano to preparative scale separations, Here's a list of the most typical types of HPLC methods and when to use Each and every.
Reduced-stream strategies are perfect for higher-sensitivity measurements of molecules in complex biological matrices wherever analyte concentrations can span numerous orders of magnitude.
This web page won't exist in the chosen language. Your choice click here was saved and you may be notified at the time a webpage is often seen in the language.